B129
Fish oil and corn oil in the prevention of colon cancer
Navneet Agnihotri1, Pooja Sarotra1, k Vidhya2, Ritu Aggarwal2, Rajat Sandhir1
1Panjab University, Chandigarh, India, 2Postgraduate Institute of Medical Education and Research, Chandigarh, India
Aim
The present study was designed to evaluate the effect of different ratios of fish oil and corn oil in the prevention of colon cancer.
Method
Male Wistar rats were divided into groups: Group-I and Group-II were fed synthetic diets and Group-III and Group-IV were fed modified diets with 1:1 and 2.5:1 ratio of fish oil and corn oil for 4 weeks. All the groups except group-I (vehicle treated) received three injections of DMH subsequently.
After sacrifice, the last 1cm of the distal colon was used for histopathology and the rest for colonocytes’ isolation. The purity of the cells was checked by measuring alkaline phosphatase. The oxidative stress was evaluated by measuring reactive oxygen species (ROS) and antioxidants.
Results
(a) The group-II animals showed lymphocytic infiltration in mucosa and submucosa with scattered lymphocytes and plasma cell formation in the crypt while, lymphoid follicle was observed in group-III. The group–IV showed mild inflammation and odema. (b) The cell viability was 60-96%. (c) The results of group-II as compared to group-I showed: (i) an increase in ROS, GSH, GST, SOD and CAT activity; and (ii) a decrease in the GPx activity. (c) Fish oil feeding led to a significant: (i) increase in levels of GSH, SOD, CAT and GPx in group–III and a decrease in group-IV as compared to group-II; and (ii) a decrease in ROS and GST in both groups III and IV.
Conclusion
This study determines the ratio of fish oil required for n-3 PUFA mediated chemoprevention. The results suggest that administration of a ratio of 2.5:1 fish oil and corn oil shows a better protection against colon cancer.
