B59
The role of KAI-1 in the adhesive, growth and invasive capacity of breast cancer cell lines
Fraz Arshad, Andrew Sanders, Wen Jiang
Cardiff University, Cardiff, UK
Background
KAI1 plays a vital role in normal physiology and certain diseases. The molecular impact of KAI-1 on cancer is also an area that requires further investigation. In recent years, a particular interest has been developed regarding its potential role as a metastasis suppressor. The aim of this study was to examine the role of KAI-1 in the aggressive nature of breast cancer cell lines.
Method
The expression of KAI-1 was reduced in the MDA-231 and forcibly expressed in the MCF-7 breast cancer cell line through transfection with a ribozyme transgene or mammalian expression construct. Alteration of the expression pattern following transfection was verified at the mRNA and protein level using Q-PCR and ICC staining. These cells were then subsequently used to assess the role of KAI-1 in the adhesion, invasion and growth of these breast cancer cell lines using a number of in vitro models.
Results
Successful knockdown of KAI-1 in the MDA-231 and KAI-1 forced expression in the MCF-7 cell line was verified. Suppression of KAI-1 in the MDA-231 cell line resulted in a significant decrease in the adhesion and a significant increase in the invasiveness and growth capacity (p < 0.05). Forced expression of KAI-1 in the MCF-7 cell line significantly increased the adhesion and decreased cell growth and invasion (p < 0.05).
Conclusion
Opposite in vitro trends were observed following the suppression and forced expression of KAI-1 in two breast cancer cell lines. This suggests that KAI-1 is involved in the adhesion, invasion and growth of breast cancer cells in vitro and may plays a role in the regulation of the metastatic spread of breast cancer cells.
