C79
Cellular expression and serum soluble forms of adhesion molecules in myeloid leukemia
Shams Ismail1, Amira Khorshed2, Hussein Gaballa2, Nahed Abdelwahab2
1National Research Center, Giza, Cairo, Egypt, 2National Cancer Institute, Cairo, Egypt
The aim of this work is to detect the expression of cell adhesion molecules, and to estimate the serum soluble forms in patients with myeloid leukemia and their correlation to clinical follow up. This study included forty-five newly diagnosed cases with myeloid leukemia. They were 32 patients with acute myeloid leukemia (AML) and 13 patients with chronic myeloid leukemia (CML). Twenty normal subjects were studied as a control group. The cellular expression of CD54, CD62L, CD56, CD44 and CD18 were estimated using the flowcytometer, and the serum level of sICAM-1, sL-selectin, sVCAM-1 and sE-selectin using the enzyme linked immunosorbent assay. sL-selectin level in cerebrospinal fluid (CSF) of AML cases at diagnosis, one month, 3 months and 6 months were also measured. In AML cases, there were significant reductions in CD54, CD62L and CD18 expression while CD44 and CD56 showed significant high expression compared to normal control group. There were significant increase in sICAM-1, sL-selectin, sVCAM-1 and sE-selectin as compared to control. CML cases showed significant decreased CD62L expression on CD34 positive bone marrow cells as compared to control and statistically increased serum sL-selectin as compared to control. With follow up, AML cases with significant high expression of CD56, low expression of CD54 and high serum level of sICAM-1 (at diagnosis) revealed resistance to treatment. CNS free cases with significant increased CSF sL-selectin level, on follow up showed CNS infiltration by leukemic cells and CNS manifestations.
