C85
S100A8 increases pancreatic and colorectal cancer cell motility and proliferation
Chin Wee Ang, Adnan A Sheikh, Sarah tonack, Paul Rooney, John P Neoptolemos, Eithne Costello
University of Liverpool, School of Cancer Studies, Liverpool, UK
Background
We have previously shown that pancreatic tumours contain S100A8- and S100A9-expressing monocytes in their stroma. We observed a similar phenomenon in colorectal tumours (submitted for publication). Here we investigate whether these S100 proteins affect pancreatic and colorectal cell motility and proliferation.
Method
Recombinant GST-tagged S100A8 and S100A9 proteins were generated in E. Coli and purified using a glutathione-sepharose column. Pancreatic (Panc-1, Suit-2 and MiaPac) and colorectal (SW480, SW837) cancer cell lines were used in the experiments. A modified Boyden Chamber assay was performed to assess cancer cell motility, whereas an MTT cell proliferation assay was performed to examine cancer cell proliferation. Experiments were carried out using GST-S100A8, GST-S100A9 or GST at concentrations ranging from 400ng/ml to 2000ng/ml, both in 1% FBS culture medium.
Results
Incubation with GST-S100A8 significantly increased pancreatic and colorectal cancer cell motility by 2- to 10-fold and 6- to 15-fold respectively compared to GST. Proliferation of these cancer cells was significantly increased in a dose-dependent manner with GST-S100A8 with the highest increase in proliferation occurring between 24 and 36 hours after incubation. The effects of GST-S100A9 on cancer cell motility and proliferation are currently being evaluated.
Conclusion
We found that S100A8 significantly increases pancreatic and colorectal cancer cell motility and proliferation. Our data indicate that this protein may play an important role in cancer development and invasion/metastasis.
