B150
Characterisation of Cytokine-mediated crosstalk between pancreatic cancer and monocytic cells
Taoufik Nedjadi, Adnan Sheikh, Chin Ang, John Neoptolemos, Eithne Costello
Division of Surgery and Oncology, Liverpool, UK
Background
We have previously shown that S100A8 and S100A9 are expressed in pancreatic cancer stroma-associated monocytes. Recently, we have observed that conditioned medias from pancreatic cancer cell lines induced expression of S100A8 and S100A9 in the HL-60 monocytic cell line. Moreover, recombinant S100A8 and S100A9 proteins were found to be potent stimulators of pancreatic cancer cell proliferation and motility.
The aim of the current study is to: 1) Investigate the potential mediators that induce S100A8 and S100A9 expression in monocytes. 2) Assess the effects of recombinant S100A8 and S100A9 proteins on cytokines secretion from pancreatic cancer cells. 3) Look at the effects of individual cytokines (such as TNF-α) on S100A8 and S100A9 expression in HL-60.
Method
Luminex multiplex kit for the detection of eight cytokines was tested on conditioned medias obtained from pancreatic cancer cell lines subjected to different conditions. Western blot was performed to analyse the expression of S100A8 and S100A9 in HL-60 cells.
Results
A number of cytokines were detected in the conditioned medias from pancreatic cancer cell lines. Interestingly, the addition of recombinant S100A8 and S100A9 proteins increased the secretion of IL-6, IL-8 and TNF-α. Furthermore, we found that TNF-α stimulated the expression of S100A9.
Conclusion
Pancreatic cancer cells produce a number of cytokines, some of which increase expression of S100A8 and S100A9 in HL-60 cells. S100A8 and S100A9 stimulate cytokines release from pancreatic cancer cell lines. This provides evidence of crosstalk between tumour cells and stromal monocytes which may contribute to pancreatic cancer growth and invasion.
