ÿþ<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <title>BACR5 - Tumour-stellate cell interaction and radiation resistance in pancreatic cancer</title> <meta name="Description" content="Mantoni Tine, Lunardi Serena, Brunner Thomas"><link href="../main.css" rel="stylesheet" type="text/css" /> <!--[if IE]> <link href="../ie.css" rel="stylesheet" type="text/css" /> <![endif]--> <script type="text/javascript" src="../js/external.js"></script> <script type="text/javascript" src="../js/sf.js"></script> </head> <body> <div id="hide">NCRI Conference Abstracts</div> <div id="container"> <div id="nav"> <img src="../images/ncri_logo.gif" alt="NCRI Logo" /> <ul> <li><a href='../pages/1.htm' title='Poster Session A'>Poster Session A </a></li> <li><a href='../pages/2.htm' title='Poster Session B'>Poster Session B </a></li> <li><a href='../pages/3.htm' title='Poster Session C'>Poster Session C </a></li> <li><a href='../pages/4.htm' title='Proffered Paper Sessions'>Proffered Paper Sessions</a></li> <li><a href='../pages/5.htm' title='Plenary Speakers'>Plenary Speakers </a></li> <li><a href='../pages/6.htm' title='Symposia'>Symposia </a></li> <li><a href='../pages/7.htm' title='Parallel Sessions'>Parallel Sessions </a></li> <li><a href='../pages/8.htm' title='Clinical Trials Showcase'>Clinical Trials Showcase </a></li> </ul> <form method="get" action="../search.htm" > <p><input name="zoom_query" value="Search..." id="searchText" type="text" /> <input value="" id="searchInput" type="submit" /></p> </form> </div> <div id="header"> </div> <div id="content"> <span class="breadCrumb"><a href='../pages/2.htm' title = 'Poster Session B'>Poster Session B</a> ...<a href='../pages/PosterMon7.htm' title='PosterMon7'>BACR Gordon Hamilton-Farley Young Investigator Award</a></span> <h1>BACR5</h1> <h1>Tumour-stellate cell interaction and radiation resistance in pancreatic cancer</h1> <h2><u>Tine Mantoni </u><sup>1</sup>,Serena Lunardi <sup>1</sup>,Thomas Brunner <sup>1</sup></h2> <h2>Gray Institute for Radiation Oncology and Biology, Oxford University, Oxford, United Kingdom <sup>1</sup></h2> <p><b>Background</b></p> <p>Pancreatic ductal adenocarcinoma (PDAC) is characterised by a strong desmoplastic reaction, which is believed to be a contributing factor to the poor therapeutic response of PDAC. A central mediator of desmoplasia is the pancreatic stellate cells (PSC). Signalling between PSC and tumour cells stimulates proliferation and migration of both cell types. We aim to gain a better understanding of the crosstalk between PSC and tumour cells and how this affects tumour cell response to ionising radiation.</p> <p><b>Method</b></p> <p>We have set up a clonogenic survival assay for tumour cells directly cocultured with PSC in order to assess the radiosensitivity of mono- versus cocultured tumour cells. We also use in vivo mouse models to study the effects of PSC on tumour proliferation and tumour regrowth in response to radiation.</p> <p><b>Results</b></p> <p>We demonstrate that PSC promote radioprotection and stimulate proliferation in pancreatic tumour cells in direct coculture. This is a PSC specific response as fibroblasts do not have a radioprotective effect on the tumour cells. Our in vivo studies of xenograft tumours in mice likewise demonstrate PSC dependent radioprotection and increased tumour proliferation. The kinetics of DNA repair in tumour cells showed no changes in the presence of PSC and was therefore concluded not to form part of this radioprotective response. We show that abrogating ²1-integrin and/or FAK signalling results in a significant decrease in the PSC mediated radioresistance in tumour cells.</p> <p><b>Conclusion</b></p> <p>We here demonstrate for the first time that PSC promote radioprotection of pancreatic tumour cells in a ²1-integrin dependent manner.</p> </div> <div id="footer"> <a class="inst" href="../instructions.html"><img src="../images/intro/instructions2.jpg" alt="Instructions" width="120" height="26" /> </a> <a class="exit" href="../exit.html"><img src="../images/intro/exit2.jpg" alt="Exit" width="120" height="26" /> </a><p class="footer">Abstract CD sponsored by the <i>BJC - Nature Publishing Group</i>.</p> </div> </div> </body> </html>